A generalized strategy for immobilizing uniformly oriented membrane proteins at solid interfaces.
نویسندگان
چکیده
We have developed a method based on self-assembly of thiols on Au substrates to immobilize membrane proteins at interfaces. Using water soluble nitrilotriacetic acid (NTA)-terminated oligo(ethylene glycol) thiols, a histidine-tagged G protein-coupled membrane receptor (GPCR) was captured in a defined orientation with little nonspecific binding.
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عنوان ژورنال:
- Chemical communications
دوره 49 26 شماره
صفحات -
تاریخ انتشار 2013