A generalized strategy for immobilizing uniformly oriented membrane proteins at solid interfaces.

نویسندگان

  • Amit Vaish
  • Vitalii Silin
  • Marlon L Walker
  • Kristen L Steffens
  • Susan Krueger
  • Alexei A Yeliseev
  • Klaus Gawrisch
  • David J Vanderah
چکیده

We have developed a method based on self-assembly of thiols on Au substrates to immobilize membrane proteins at interfaces. Using water soluble nitrilotriacetic acid (NTA)-terminated oligo(ethylene glycol) thiols, a histidine-tagged G protein-coupled membrane receptor (GPCR) was captured in a defined orientation with little nonspecific binding.

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عنوان ژورنال:
  • Chemical communications

دوره 49 26  شماره 

صفحات  -

تاریخ انتشار 2013